JAZMINE A.

Title:

Introduction:

Materials & Methods:

Results:

Figure 1: Bacterial protein expression shown without Amp., organism (BL21(DE3)), DNA (pGEM-gbr22). This was considered a "Fun Plate" to see if there was bacteria from gloves.




Figure 2: Bacterial protein expression control plate. It has Amp., BL21(DE3), but no DNA. No bacterial growth.



















Figure 3: Bacterial protein expression experimental plate. It has Amp., BL21(DE3), and DNA (pGEM-gbr22). Purple colonies were beginning to show.




Figure 4: Bacterial protein expression. Purple culture in erlenmeyer flask with Amp., BL21(DE3), DNA (pGEM-gbr22).




Figure 5: Bacterial protein expression. purple cell pellet with Amp., BL21(DE3), DNA (pGEM-gbr22).




Figure 6: Protein purification. Elution 1 and Elution 2 with purified protein.




Figure 7: Protein purification. Spectrum of elution 1 at 280 nm of gbr22 purified protein





Figure 8: Protein purification. Spectrum of elution 2 at 280 nm of gbr22 purified protein.




Figure 9. Protein purification. Spectrum of elution 1 at 574 nm of gbr22 purified protein.




Figure 10: Protein purification. Spectrum of elution 2 at 574 nm of gbr22 purified protein.




Figure 11: Protein characterization. Gel with bands of cell lysate, soluble fraction, flow through, wash, elution 1, and elution 2.





Discussion:

Conclusions:

References: